EpiMelt TWIST test assay
Homo sapiens twist basic helix-loop-helix transcription factor 1 (TWIST1), NM_000474
DNA Methylation Assay Kit for TWIST, based on MS-HRM. Includes controls
Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of TWIST templates supplied with the MethylDetect TWIST kit.
Results obtained following the MethylDetect TWIST kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.
Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of TWIST templates supplied with the MethylDetect TWIST kit.
Results obtained following the MethylDetect TWIST kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.
