EpiMelt ONECUT2 test assay
Homo sapiens one cut homeobox 2 (ONECUT2), NM_004852
DNA Methylation Assay Kit for ONECUT2 based on MS-HRM. Includes controls
Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of ONECUT2 templates supplied with the MethylDetect ONECUT2 kit.
Results obtained following the MethylDetect ONECUT2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.
Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of ONECUT2 templates supplied with the MethylDetect ONECUT2 kit.
Results obtained following the MethylDetect ONECUT2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.