EpiMelt BRCA2 test assay
Homo sapiens breast cancer 2, early onset (BRCA2), NM_000059
DNA Methylation Assay Kit for BRCA2, based on MS-HRM. Includes controls
Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA2 templates supplied with the MethylDetect BRCA2 kit.
Results obtained following the MethylDetect BRCA2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.
Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of BRCA2 templates supplied with the MethylDetect BRCA2 kit.
Results obtained following the MethylDetect BRCA2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.