Resources
Resources
Resources for MS-HRM
Here you will find some of our resources concerning MS-HRM:
Application Notes
Application Note: Bisulfite Modification Standard Kit
High bisulfite conversion efficiency using EpiMelt Bisulfite Modification Standard Kit on genomic DNA…
Application Note: EpiMelt Real-Time PCR Master Mix
High-sensitivity EpiMelt DNA methylation analysis using EpiMelt Real-Time PCR Master Mix…
Protocols
Protocol: Bisulfite Modification Standard Kit
Complete kit for efficient conversion and preparation of converted DNA for methylation research…
Protocol: Assay Protocol
The performance of the EpiMelt Methylation Detection Assay has been tested on the…
Protocol: EpiMelt Real-Time PCR Master Mix
High specificity ready-to-use for real-time Hot Start PCR with EvaGreen®. Optimized for epigenetic…
Other
What Is Epigenetics?
Epigenetics is a study of the heritable gene expression changes that do not involve alternations in DNA sequence…
What Is Bisulfite Modification?
During DNA replication (cell division) methyl groups (DNA methylation) are resynthesized on the newly replicated…
What Is DNA Methylation?
Enzymatic addition of methyl groups (CH3-) to the DNA molecule is referred to as DNA methylation…
What Is DNA Melting?
Dissociation of the double stranded DNA helix into single coils is referred to as DNA melting…
How Does MS-HRM Work?
The number of cytosines (C) in a PCR product, amplified from a sodium bisulfite modified template, depends on the…
PCR Bias In Methylation Experiments
The methylated and non-methylated locus after bisulfite modification differs in number of cytosines…
Controls In Methylation Detection Experiments
Following good laboratory practice any experiment needs to be properly controlled…
MS-HRM For Methylation Detection In FFPE Material
Formalin-Fixation followed by paraffin-embedment (FFPE) is one of the most widely practiced methods for…
MS-HRM For Methylation Detection In Liquid Biopsies
Tumor DNA is secreted into body fluids that come in direct contact with the tumor site…
DNA Methylation In Cancer
Methylation of mammalien cell DNA is the most studied, well-established epigenetic mechanism…
Methylation Diabetes
The epigenome is determined by DNA methylation and histone modifications, which together is…
Methylation In Lung Cancer
Lung cancer is the leading cause of cancer related deaths world wide. Often patients with lung cancer…
MGMT Gene
O6-methylguanine-DNA methyltransferase (MGMT) is a DNA repair enzyme, involved in cellular…
Methylation Glioblastoma
Gliomas are the most common neoplasm of the central nervous system, they originate from glia cells…
Methylation Liver Cancer
Liver cancer is one of the most lethal cancers worldwide. It has a highly aggressive disease course…
MLH1 Gene
The MutL homolog 1 (MLH1) gene is a tumor suppressor gene involved in the DNA mismatch…
BRCA2 Gene
The tumor suppressor gene BRCA2 encodes the BRCA2 protein, which is involved in…
BRCA1 Gene
The breast cancer type 1 susceptibility gene (BRCA1) is a tumor suppressor gene involved in many…