EpiMelt SHOX2 test assay
Homo sapiens short stature homeobox 2 (SHOX2), NM_003030
DNA Methylation Assay Kit for SHOX2 based on MS-HRM. Includes controls.
Normalized melting curves illustrating positive control (red), the assay calibration control (blue), and negative control (orange) of SHOX2 templates supplied with the MethylDetect SHOX2 kit.
Results obtained following the MethylDetect SHOX2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.
Relative signal difference (d/dT) plot illustrating the positive control (red), the assay calibration control (blue), and negative control (orange) of SHOX2 templates supplied with the MethylDetect SHOX2 kit.
Results obtained following the MethylDetect SHOX2 kit protocol, using the EpiMelt Real-Time PCR Master Mix. After PCR, the amplicons were analysed by high resolution melting curve analysis, and the data evaluated using the LightCycler® Gene scanning software.
